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If the solution is not clear, it may be a sign of contamination or degradation, and we recommend not using it
A study from north-west India
and P.M.K
Western blot analysis confirmed that the FUS-RRMmut fly line expresses almost an equivalent level of the FUS proteins to the FUS fly line (Figure 2figure supplement 1)
The blocked membranes were incubated with the specific primary antibodies diluted in 5% BSA (Sigma-Aldrich) with 0.05% sodium azide overnight on a shaking bed at 4 C
Tojo et al., 2005
