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Calorie restriction or exercise also increases tissue NAD+ content thereby activates SIRT1, which then activates PGC-1 through deacetylation [178]

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Effect of Circadian Rhythm on Metabolic Processes and the Regulation of Energy Balance

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Cells were resuspended in 100 l postnatal cortical neuron culture medium, and live cells were quantified using trypan blue (1450021

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However, the detailed mechanism needs to be further investigated

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