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Cayman Chemical, Ann Arbor, MI, USA) according to the manufacturers instructions

The failure of Lys487 to form hydrogen bonds with Arg264 in its own subunit (or with Arg475 in its dimer subunit) results in a weaker NAD + binding site, a situation that ultimately affects the kinetic properties of the enzyme (Larson et al., 2007)

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The analysis of the 10-year period showed an increasing trend in male prevalence, which must be analyzed considering that if, on the one hand, candidates for blood donation are predominantly men, on the other hand, this preponderance has always been the same in the blood banks in an epidemiological context of higher prevalence of HTLV-1/2 in female blood donors