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Advances in IgA glycosylation and its correlation with diseases

After 12 h, cells were washed with D-PBS to remove the complete medium and loaded with 40 L of 10 M coelenterazine 400a (Maokangbio) diluted in the assay buffer (HBSS containing 0.01% bovine serum albumin and 5 mM HEPES, pH 7.5) per well

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