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The resin was washed three times with 500 L of wash buffer (50 mM Tris-HCl (pH 8.0), 150 mM NaCl, 20 mM imidazole, and 0.03% DDM), and bound proteins were eluted with 200 L of elution buffer (50 mM Tris-HCl (pH 8.0), 100 mM NaCl, 250 mM imidazole, and 0.03% DDM)

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Of note, NF-B-mediated inflammation is often via the inflammasome and is associated with many white matter associated diseases and is upregulated in the aged brain (Tilstra et al., 2011

doi: 10.1016/j.freeradbiomed.2007.05.026

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Association between serum 25-hydroxyvitamin D level and upper respiratory tract infection in the third national health and nutrition examination survey