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PMID 27468410

By staying in the middle zone and targeting the outer surface, you minimize the chance of encountering significant blood vessels

[DOI] [PMC free article] [PubMed] [Google Scholar] 18.El-Haschimi K, Pierroz DD, Hileman SM, Bjrbaek C, Flier JS

In both male and female GHRKO mice, disruption of the GH/IGF-1 axis from birth results in mice that are remarkably long lived, but temporal disruption of this pathway in young adults (aGHRKO mice) improves lifespan only in females 17 , an observation which is consistent with the beneficial effects of targeting IGF-1R signaling post-development on female healthspan and aging in this study

This can stimulate angiogenesis at sites of tissue disruption in animal models ERK1/2 activation: Studies in preclinical settings have observed activation of extracellular signal-regulated kinases

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