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The oil that separates is extracted with ether, the extract dried over anhydrous sodium sulfate and then evaporated at reduced pressure

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To be used for the experiments, the lyophilized powder was initially dissolved in NH 3 OH 6 M to obtain a stock solution with a final concentration of 250 M

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However, if antioxidant defense systems are severely impaired or the more realistic scenario that an iron supplement is co-ingested, the mode of cell death can be switched to ferroptosis