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Air-dried smears were fixed in Carnoys solution (methanol: acetic acid, 3:1) for 2 h, stained with AO solution (0.19 mg/mL AO in phosphate-citrate buffer, pH 2.5) for 5 min, and immediately examined under a fluorescence microscope

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CD47, an integral membrane receptor, serves as an inhibitory signal that mitigates phagocytosis by binding to the macrophage receptor SIRP

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