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In case the study is on humans, the sample size must be calculated to achieve a statistical power of at least 80% [473]
Statistical analysis was performed using GraphPad Prism 5 software, where comparison of two means utilized an unpaired Student's t- test, and comparison of multiple means used a one-way analysis of variance (ANOVA) with a Tukey post-test to compare two variables where applicable

However, specific interaction studies are limited, so conservative approaches are advisable

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