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For cell culture studies, 6AN (6-aminonicotinamide), trolox ([]-6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid), H89 dihydrochloride hydrate (N-[2-(p-Bromocinnamylamino)ethyl]-5-isoquinolinesulfonamide dihydrochloride), forskolin, buthionine sulfoximine (BSO), and N-acetyl cysteine were dissolved in water