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Endocrine and metabolic effects of long-term administration of [Nle27]growth hormone-releasing hormone-(1-29)-NH2 in age-advancing men and women

The comparative threshold cycle (Ct) method was used to measure the relative expression, where 2 Ct represents the fold change in expression, as previously described (Livak and Schmittgen, 2001)

FIMO [ 33 ] was used to scan for motifs in the promoter regions of all transcripts in the transcriptome using the parameters no-qvalue thresh 1e4

doi: 10.1016/s0882-4010(03)00048-2

Cell lysates were extracted using the indicated dose of NaCl buffer and analyzed by immunoblotting (top) and CBB (middle)

Amino acid transporter SLC7A11/xCT at the crossroads of regulating redox homeostasis and nutrient dependency of cancer