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Description
WAT mitochondria isolation To isolate mitochondria, fresh gWAT was minced then homogenized using a drill-operated Teflon pestle in ice-cold MSHE buffer (70 mM sucrose, 210 mM mannitol, 5 mM HEPES, 1 mM EDTA, pH 7.2) containing 0.5% FA-free BSA

Blood vacuum containers for full blood, plasma and serum where customized by adding either NEM or PBS (control tubes) prior to being employed for collecting blood from 5 healthy volunteers
Cystine-free/GSH-supplemented = 6

(1) Radiation sickness and radiation safety: radiation, radioactive substances or because of leukopenia caused by anticancer drugs and other symptoms can play a protective effect

PMID: 27456681 Lobo, V

Garlic's active principle, allicin, is thought to contribute significantly to the compound's antioxidant properties (Ernsberger, Johnson, Rosenthal, Mirelman, & Koletsky, 2007)