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up to 70% of fALS cases are majorly attributed to four genes: C9orf72, SOD1, TARDBP, and FUS (29), while other ALS-causing genes and their roles are enlisted in Table 2

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Western blotting For whole-cell protein extraction, cell pellets were lysed in cold RIPA buffer (50 mM TrisHCL pH 7.4, 5 mM EDTA, 250 mM NaCl, 50 mM NaF, 0.1 mM Na 3 VO 4 , 0.1% Triton X-100), supplemented before use with cOmplete Protease Inhibitor Cocktail (Roche) and 5 mM sodium butyrate

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25 This process not only clears necrotic debris but also advances liver repair, opening new avenues for therapeutic strategies in liver repair and regeneration