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Denatured samples (100C for 5 min) were separated by SDS electrophoresis, blotted onto a nitrocellulose membrane (Amersham Protram 0.2 m PC, GE Healthcare Life Sciences, United States), and incubated with specific polyclonal antibody at the following concentrations: anti-AOX (Agrisera, Sweden) 1:100, anti-NgDJ-1 1 1:50, and anti-NfNDH-2 1:1,000

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ZhuLLiJWeiCLuoTDengZFanYet alA polysaccharide from Fagopyrum esculentum Moench bee pollen alleviates microbiota dysbiosis to improve intestinal barrier function in antibiotic-treated mice

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Spinal stenosis ranks as another frequent cause, particularly in adults over 50 this narrowing of the spinal canal puts pressure on the nerve endings that form the sciatic nerve