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The residue was purified using preparative HPLC (column: Gemini 5 M NX-C18 110 LC-column 50 21.2 nm, elution: 3373% 10 mM NH 4 CO 3 in water/CH 3 CN)

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(2023) 19:e2303459

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Variations in Study Results and Inconsistencies The efficacy of Astragalus membranaceus and its active compounds varies across studies due to differences in the following: Extract composition and standardizationMany studies use different extraction methods, leading to variability in the concentration of bioactive compounds