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Once opened, refrigeration is usually recommended to prevent microbial growth, even if the unopened product did not require cold storage

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While intramuscular (IM) injections are widely used and generally safe when performed properly, even a small error in technique, needle placement, or injection site selection can lead to significant medical complications

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All cell lines were regularly tested for mycoplasma contamination using MycoAlert Mycoplasma Detection Kit (Lonza) and by assessment of (lack of) DAPI-positive microvesicles under fluorescence microscope