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Description
Cells plated in glass-bottom 96-well plates were treated for the indicated times and, after fixation with 4% paraformaldehyde, images were acquired using a high-content microscope (Operetta, Perkin-Elmer)
It can take weeks (or even months) to assess whether a product has been effective in lightening dark spots
They can highlight the cheeks, plump the lips, refine the jawline, or soften folds for a balanced, natural result
Desalt the supernatant with PD-10 columns equilibrated with 100 mM NaCl containing 20 mM Na + /K + phosphate buffer pH 7.4 (or 20 mM Tris base for rat RBCs)
Burgering BM, Coffer PJ
doi: 10.1007/s11033-023-08839-x
