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pH-Channeling in Cancer: How pH-Dependence of Cation Channels Shapes Cancer Pathophysiology

HED/Cys-SG and CHP assays If not otherwise indicated, strep-MBP-GRX samples were reduced with 10 mM DTT for 2 hours and the buffer was exchanged against 100 mM Tris/HCl p H 8.0, 1 mM EDTA 15 using PD MiniTrap G-25 desalting columns (GE Healthcare)

2024;384:e077169

Okuno S, Sato H, Kuriyama-Matsumura K, Tamba M, Wang H, Sohda S, et al

Protein stability is particularly important in erythrocytes since they are anucleate with a 120-day lifespan devoid of protein synthesis
