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10.1016/j.ecoenv.2024.117479 104 RadchenkoM

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Mice were perfused with cold phosphate-buffered saline (PBS)heparin (5 IU ml 1 ) via the left ventricle at a controlled pressure of 150 mmHg for 6 min to exsanguinate the kidneys or brains before removal

These estimated half-lives for protein-SOHs are on par with the cellular lifetimes for many other post-translational modifications, including phosphorylation and O-GlcNAc (the half-lives for some of which are estimated to be less than 1 min) [188]

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