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An aliquot of 5 l of this reaction was diluted to 180 l with reaction buffer (100 mM Tris (pH 8.0), 1.5 mM EDTA, 1 mM DTT) to attain final concentrations of 5 g ml 1 GSTO1 and 0.25 IC 50 of inhibitor

However, as the oxidative stress increases and the balance between GSH and GSSG shifts toward GSSG, more cysteines are lost from the system per hour

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