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The tissues were blotted on filter paper, weighed, and homogenized in 0.05 M phosphate buffer (pH7.5) using a tissue homogenizer at 40C

Semin Cancer Biol 75:314 Kim I, Shu CW, Xu W, Shiau CW, Grant D, Vasile S et al (2009) Chemical biology investigation of cell death pathways activated by endoplasmic reticulum stress reveals cytoprotective modulators of ASK1

Most patients undergo two sessions per week for three weeks, followed by a maintenance plan

J Nutr Biochem 107:109034

If there is no response after the first injection, wait 24 hours and increase the dose by 2.5-10mcg