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Then the supernatant was collected, lyophilised and stored at -20C before use

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Furthermore, the ALDH1A1 inhibitor DSF and chemotherapeutic agent gemcitabine cooperatively inhibited breast tumor growth and tumorigenesis by purging ALDH + TICs and activating T-cell immunity (Liu et al., 2021)

Protein was extracted from SH-SY5Y cells, N2A cells and mouse cortex using CellLytic buffer (Sigma) per manufacturer's instructions, separated by electrophoresis and blotted to PVDF membranes

therefore, additional study is essential to identify effective therapeutic strategies targeting various pathways, including 7nAChRs

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