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Bound proteins were released by boiling the beads in sample buffer and then identified by SDS-PAGE under denaturing conditions and immunoblotting with selected antibodies

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Indeed, the skin appears to be an immunological battleground with elevated levels of IL-1, IL-6, and TNF

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However, interestingly, deletion of the glucocorticoid receptor specifically in Osterix-expressing bone marrow osteoprogenitor cells increases BMAT and reduces cortical and trabecular bone mass under ad libitum feeding, calorie restriction conditions, and with aging (390, 391), suggesting that excessive or deficient glucocorticoid signaling in the BMAd progenitors can lead to similar outcomes