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The resulting supernatant was then transferred to new tubes and stored in a 80C freezer

doi: 10.1007/s00394-014-0706-z, PMID: [DOI] [PubMed] [Google Scholar] 28.Izumi H, Sato K, Kojima K, Saito T, Saido TC, Fukunaga K

S.ButersJ

Alternatively, if the vial has positive pressure, pushing water in becomes difficult because you are compressing the air inside

Since HQ requires a reducing agent in order to become a TyrBm substrate, the kinetic parameters of the monophenolase activity were determined in the presence of H 2 O 2

This can lead to contamination in medical settings and so using bacteriostatic water as part of the process helps to ensure that no biofilm forms on the peptides being reconstituted
