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MLE-12 cells (ATCC, CRL-2110) were grown in DMEM/F12 (Gibco) supplemented with 2% FBS, penicillin (100 U/mL), and streptomycin (100 mg/mL)

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doi: 10.1038/s41392-025-02228-5 103 SweeneyCVyasP

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Consequently, the genetic circuit is activated only in regions exhibiting both low oxygen and high lactate, hallmarks of the TME

Sugino T, Hasegawa Y, Kurose Y, Kojima M, Kangawa K, Terashima Y (2004) Effects of ghrelin on food intake and neuroendocrine function in sheep