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The most devastating consequence of this impaired lipid clearance is accelerated atherosclerosis, which manifests as premature coronary artery disease and myocardial infarction, often occurring before age 50

Adherent cells were scrapped, then centrifuged at 4C and 13000 g for 15 min, and the resulting supernatants processed using established protocols.[19] Intracellular levels of 1-MNA and as well as the IS were determined from LC/MS/MS peak areas