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The mean weighted des was obtained by performing a bi-exponential fit (Chebyshev algorithm, built-in Clampfit 11.2, Molecular Device), which was used to calculate mean desensitization by using: (A1* 1 + A2* 2)/(A1 + A2) A1 = Amplitude (initial magnitude) of the first exponential component 1 = Time constant of the first component A2 = Amplitude of the second exponential component 2 = Time constant of the second component To study recovery from desensitization, a paired-pulse protocol was employed in which the amplitude of the test pulse was normalized to that of the preceding desensitizing pulse and plotted against the time interval between the paired pulses