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Membranes were washed three times using Tris-buffered saline-Tween20 (TBST) and subsequently incubated with anti-rabbit or anti-mouse IgG (H + L

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By disrupting energy metabolism and antioxidant defenses in these immune cells, heavy metals promote a state of unresolved inflammation that contributes to neuroimmune dysregulation and chronic symptoms such as fatigue, brain fog, and heightened chemical sensitivity

The structure for ligands were downloaded from the ChEBI database (www.ebi.ac.uk/chebi/ init.do) using CHEBI id, CAS numbers (www.chemicalbook.com) or their PDB id code (www.ligand-expo.rcsb.org)