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Glutamate: Its role in learning, memory, and the aging brain

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Add bacteriostatic water slowly down the vial wall (not directly onto powder)
The fused macrocyclemRNA was subsequently reverse transcribed by RQ-RTase (Promega) for 1 h at 42 C and incubated with human recombinant Fc protein-immobilized Dynabeads protein G (Life Technologies) for 30 min at 4 C (negative selection against Fc-tag and beads)

Anderson, M