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To this end, a 96-well Nunc Maxisorp plate (Thermo Fisher Scientific) was coated with 5 g/mL human CEA-Fc chimera (Genscript, Piscataway, NJ) or HER2 extracellular domain (EMP Genetech, Ingolstadt, Germany) in PBS (4 mM KH 2 PO 4 , 16 mM Na 2 HPO 4 , 115 mM NaCl, pH 7.4) at 4 C overnight and blocked with 3 % (w/v) bovine serum albumin (BSA) in PBS/T (PBS supplemented with 0.1 % (v/v) Tween-20) for 2 h at room temperature
NO activates sGC, elevating cGMP levels, which, in turn, activate PKG
It exists as a pre-formed heterotetramer of two extracellular alpha subunits and two transmembrane beta subunits linked by disulfide bonds
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