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Real-time quantitative reverse transcription PCR (RT-PCR) was employed to assess the expression levels of Wnt7a and Wnt10a genes

N.YanceyK.PodlutskayaV.NagykaldiE.GautamT.et al (2017)

It is also reported that NAC may improve the function of endothelial cells lining blood vessels [82]

[DOI] [PubMed] [Google Scholar] 40.Nuttall S.L., Khan J.N., Thorpe G.H., Langford N., Kendall M.J

Cytokines leak into the circulation quickly after direct intratumoral injection, leading to a short retention time at the tumor sites, while mRNA is able to achieve long-lasting cytokine expression

Proper sterile procedure is absolutely non-negotiable