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Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) According to the protocol, total RNA was extracted from the large intestine with TRIzol (Life Technologies Japan Ltd., Tokyo, Japan) using a Dounce Tissue Grinder (DWK Life Sciences, LLC, NJ, USA), and 500 ng of RNA was reverse transcribed to complementary DNA using the Super Script III First-Strand Synthesis Super MIX kit (Life Technologies Japan Ltd.)

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doi: 10.1016/j.neo.2021.11.002 92 HouessinonAFranoisCSauzayCLouandreCMongelardGGodinCet al

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Storage Conditions That Impact Shelf Life To maximise shelf life (both lyophilised and reconstituted) you should pay attention to the following key storage parameters: Temperature Lower temperatures slow chemical reactions