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Notably, we observed the delipidation of VIT-2::GFP-containing particles in embryos with NR 2p-FLIM (separate GFP and Nile Red signals in the embryos shown in Supplementary Figure S5), in agreement with the previous BCARS finding ( Under low-concentration Nile Red vital staining conditions, most of the Nile Red molecules have been reported to label acidic gut granules rather than lipid particles in the intestine (Supplementary Figure S3)

Information on the primers used is presented in Supplementary Table 1