glutathione e.coli expression The System: A Journey from Cyanobacteria to Higher Eukaryotes Recombinant Protein: E. coli-Based Expression

2.3 Randomization Participants were randomized (1:1) using a pre-specified block randomization sequence (BABABAABAABBABBA), ensuring balanced allocation across treatment sequences

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The denatured AP (400 L) was loaded onto a 30 kD spin column, and the samples were centrifuged at 14,000 g at RT for 15 min, leaving 40 L of sample