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Abbreviations -HCCA: -Cyano-4-hydroxycinnamic acid DAD: Diode array detector DHB: 2,5-Dihydroxybenzoic acid DTNB: Ellmans reagent (5,5-dithio-bis-2-nitrobenzoic acid) DTT: Dithiothreitol GCMS: Gas chromatographymass spectrometry GSH: Reduced glutathione GSSG: Oxidized glutathione HPLCMS/MS: High performance liquid chromatographytandem mass spectrometry MALDI-TOF/MS: Matrix-assisted laser desorption with ionization time of flight MRM: Multiple reaction monitoring MS: Mass spectrometry NSECM: N , S -Ethoxycarbonyl methyl esters ROS: Reactive oxygen species SIM: Single ion monitoring tGSH: Total glutathione TFA: Trifluoroacetic acid References Anto M, Arnau S, Buti E, Cortijo V, Gutirrez E, Sol M (2009) Ecotoxicol Environ Saf 72:14551462 Balzer I, Espinola IR, Fuentes-Pardo B (1997) Biol Cell 89:539543 Bjerregaard P, Christensen L (1993) Mar Ecol Prog Ser 99:271281 Bouligand J, Deroussent A, Paci A, Morizet J, Vassal G (2006) J Chromatogr B 832:6774 Camera E, Picardo M (2002) J Chromatogr B 781:181206 Capitan P, Malmezat T, Breuill D, Obled C (1999) J Chromatogr B 732:127135 Circu ML, Aw TY (2008) Free Radic Res 42:689706 Cohn VH, Lyle J (1966) Anal Biochem 14:434440 Ellman GL (1959) Arch Biochem Biophys 82:7077 Fanjul-Moles ML, Prieto-Sagredo J, Lopez DS, Bartolo-Orozco R, Cruz-Rosas H (2009) Photochem Photobiol 85:7887 Filho DW (1996) Braz J Med Biol Res 29:17351742 Frassanitoa R, Rossi M, Dragani LK, Tallarico C, Longo A, Rotilio D (1998) J Chromatogr A 795:5360 Green JM (1996) Anal Chem 68:305A309A Guan X, Hoffman B, Dwivedi C, Matthees DP (2003) J Pharm Biomed Anal 31:251261 Hissin PJ, Hilf R (1976) Anal Biochem 74:214226 Hjeltnes B, Samuelsen OB, Svardal AM (1992) Dis Aquat Org 14:3133 Hottenroth S, Vlkel W, Fechner T, Bopp S, Rmpp A, Klemm O, Frank H (2000) 1st International Symposium on Advances in Chromatographic and Electrophoretic Separations (ACES), Bayreuth Humbert B, Nguyen P, Obled C, Bobin C, Vaslin A, Sweeten S, Darmaun D (2001) J Mass Spectrom 36:726735 Humbert B, Nguyen P, Martin L, Dumon H, Vallette G, Maugre P, Darmaun D (2007) J Nutr Biochem 18:1016 Iwasaki Y, Saito Y, Nakano Y, Mochizuki K, Sakata O, Ito R, Saito K, Nakazawa H (2009) J Chromatogr B 877:33093317 Ji LL, Leeuwenburgh C (1995) Glutathione and exercise

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Mahmood S, Inada N, Izumi A, Kawanaka M, Kobashi H, Yamada G