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Harvested and washed with 1 ice-cold Phosphate Buffered Saline (PBS)- cell pellets were resuspended in 0.02 M Sodium Phosphate Buffer pH 7.2, disrupted by mild sonication, and then centrifuged at 1000 g for 5 min, at 4 C, to remove cell debris

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The pred seems to have helped take the severity out of it (not sure what will happen when i finish that up though)

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