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(3) Ultrasonic extraction in an ice-water bath for 10 min, and leave at -40C overnight

Discontinuing at the first sign of shedding may prevent the regrowth that would have followed

People shake the vial like a cocktail mixer to dissolve the powder faster

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[DOI] [PMC free article] [PubMed] [Google Scholar] 142.Constant SL, Bottomly K

Zheng XT, Yu ZC, Tang JW, Cai ML, Chen YL, Yang CW et al (2020) The major photoprotective role of anthocyanins in leaves of Arabidopsis thaliana under long-term high light treatment: antioxidant or light attenuator