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Although these studies in GF mice have been important in providing evidence supporting the concept that the microbiome is involved in brain processes involved in stress hormone signaling, neural function and neuroprotection 51 , there are significant limitations to human translation of these findings including, but not limited to, the presence of defects in the immune system development, ENS formation and CNS maturation of GF animals 57,41,4248,49, 50

The compound has been studied across multiple tissue contexts (tendon, ligament, muscle, gastrointestinal, vascular)

The same procedure was done in parallel, except the NSP r was omitted from the Assembly Buffer and 0.02% DDM was included in Buffer A, B, and C

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