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After reaching confluency, the cells were subcultured by trypsinization at a 1:3 dilution ratio

doi: 10.1165/rcmb.2013-0382PS

Interpreted the results: CC and LL

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WZ: conceptualization, funding acquisition, validation, and writingreview and editing

GHK-Cu is a robust complex, but the biochemical environment of the human nasal mucosa (pH variations, mucociliary clearance) poses physical and enzymatic barriers not fully modeled in murine anatomy