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Upon activation of PI3K/AKT cue, activated PI3K phosphorylates and activates AKT, with consequent activation of cyclic adenosine monophosphate (CAMP) response element-binding protein (CREB), which enhances the generation of BDNF (Luo et al

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& Amital, H

We measured the yield of this purification protocol using SpyTag002-sfGFP and fluorescence measurements of samples on SDS PAGE gels, in order to quantify only the coupling products and not any protein contaminants that are purified away during the procedure