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In vivo metal selectivity of metal-dependent biosynthesis of cobalt-type nitrile hydratase in Rhodococcus bacteria: a new look at the nitrile hydratase maturation mechanism

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To ensure equal seeding density across wells, CFU validation was performed by plating aliquots on Middlebrook 7H11 agar and enumerating colonies after a 4-week incubation at 37 C