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Combining SEC with ultracentrifugation can enhance EV enrichment, purity, and integrity ( 3.7 Recent advances in scalable isolation Recent protocol optimizations demonstrate promising pathways toward GMP-compatible manufacturing of MEVs

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Compounds like acetate and succinate that are used in the oxygen respiration cannot be used when TMAO is terminal electron acceptor (DiChristina and DeLong, 1994) and on the contrary, acetate is a product of the anaerobic TMAO reduction (Ringo et al., 1984

& Ehrenstein, M

Mobile phase A consisted of 10 mM ammonium acetate in water (pH 9) with a 5 M Agilent InfinityLab deactivator additive (p/n 5191-4506), and mobile phase B consisted of 10 mM ammonium acetate (pH 9) in 10:90 (v:v) water/acetonitrile with a 5 M Agilent InfinityLab deactivator additive (p/n 5191-4506)