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Briefly, genomic DNA was collected from 1 10 6 cells using the Puregene Cell Kit (Qiagen)

The activation of GRB2 results in signal transduction via the activation of the guanine nucleotide exchange factor, SOS (son of sevenless encoded by the SOS1 gene) which in turn activates the monomeric G-protein, RAS

We previously reported that the CRL4B/NuRD (MTA1) complex is recruited by transcription factors such as SNAIL and ZEB2 to jointly bind to the promoters of E-cadherin and AXIN2, promoting cell invasion and stemness [14]

10.1073/pnas.1509158112 70 KempK.RedondoJ.HaresK.RiceC.ScoldingN.WilkinsA

ACC 57) at passage 425 were cultured in Dulbeccos Modified Eagle Medium (DMEM) (Gibco, Ref: 41966029, Thermofisher Scientific,Waltham, Massachusetts, United States) supplemented with 10% foetal bovine serum (FBS) (Gibco, Ref: 10270106, Thermo Fisher Scientific, Waltham, Massachusetts, United States) in humidified air at 37 C and 5% CO 2

10.1038/nature08221 38 HartR