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The oocytes were analyzed using fluorescence microscopy (Eclipse 80i, Nikon Instruments Europe, Amsterdam, The Netherlands) at 20 magnification with an Ex filter of 475/30 nm, a DM 405 nm, and an Em filter of 530/40 nm

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Goal-based phase shifting Advanced researchers shift peptide focus based on phases
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Seino Y, Nanjo K, Tajima, et al
