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Crystallization and Structure Determination The isopeptide bond-containing N13K/V26F/Q67E/P117N quadruple mutant of FctB (FctB-iso) was subjected, as a fusion protein with MBP (described above), to initial crystallization experiments by sitting drop vapor diffusion at 18 C using a Cartesian nanoliter dispensing robot (Genomic systems) and a locally compiled crystallization screen with 480 conditions 35

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Quillan, K

10.1016/j.toxicon.2023.107094 74 ParkK

Similarly, ROS-induced ER stress triggers the unfolded protein response (UPR), which leads to further cell apoptosis and the immune system targeting melanocytes 56