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It is synthetically produced and must be converted into active forms that the body can readily use

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Protective effect of brain-derived neurotrophic factor on pancreatic islets in obese diabetic mice

For colocalization immunofluorescence staining, the slides were incubated with Alexa Fluor conjugated secondary antibody (Invitrogen, #Alexa Fluor 568-A11011, Alexa Fluor 488- A11029) for 2 h at room temperature