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While subcutaneous injection into the lower limbs may be possible, it is generally not recommended as a preferred injection site due to the possible complications involved including tissue damage and injection site reactions: The skin in the lower limbs is typically thinner and more sensitive than other parts of the body, which can increase the risk of pain, bleeding, and infection

Blood vessel formation begins

So we just use an insulin needle and it's just superficial injections on either side of my incision, just as close to my Achilles as possible

Cell proliferation was assessed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetra-zolium bromide (MTT

doi: 10.1089/jmf.2018.0024

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