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Measurements were validated using the Wave software (Agilent, Santa Clara, California, USA) and normalized to DNA concentrations per well, analyzed in stained cells (Hoechst 33342) using a plate reader (Tecan Infinite M200, Tecan Group Ltd., Mnnedorf, Switzerland) with an excitation wavelength of 350 nm and an emission wavelength of 461 nm

In cell culture studies, the decrease in oxidative stress by acetylcarnitine occurred by 1) exerting a protective effect on mitochondrial structure and function, making the ETC less prone for electron leak and superoxide production, and 2) stimulating the endogenous cellular antioxidant defence mechanisms

However, both groups had a low baseline pain level, which limited the ability to distinguish differences after treatment

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