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Twenty-four hours later, to test sociability, a stranger rat (Stranger 1) was placed inside a small plastic cage with mesh-like holes in one side chamber and an empty cage in the third chamber

For the electron flow assays, mitochondria were plated in MAS buffer with 10 mM pyruvate + 2 mM malate + 4 M FCCP followed by 20 M rotenone in port A, 100 mM succinate in port B, 40 M antimycin A in port C and 1 mM TMPD + 100 mM ascorbic acid in port D

Nature 569:270274

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Finally, we thank Dr

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