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Biochem 2019, 93, 273289

Malaguarnera, M

4f, 7f) The same arbitrary threshold (108-65535) was applied to a single frame (beginning of cleavage furrow ingression) of the original time-lapse images of 5 s interval for both control and mutant embryos, expressing PI(4,5)P 2 biosensor, mCherry::PH PLC1 , to segment PI(4,5)P 2 -positive membranes from the background (pixel size cut-off: 5-infinity)